3,3-二氨基联苯胺 四盐酸盐 水合物(DAB) ≥96%

DAB (3-3′ diaminobenzidine tetrachloride) is suitable for immunoblotting and immunohistochemical staining of nucleic acids and proteins. In HRP-based assays, the buffer oxidizes to a brown polymer in the presence of HRP and hydrogen peroxide that is insoluble in most organic solvents; sites of HRP activity on tissue sections and blots appear as brown-orange deposits, which can be intensified by treatment with salts of silver, copper, nickel, cobalt and osmium.