CD86 type I membrane protein is a member of the immunoglobulin superfamily which is expressed by antigen-presenting cells, and acts as the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. CD86 binding with CD28 protein is a co-stimulatory signal for initiation of the T-cell. CD86 binding with CTLA-4 negatively regulates T-cell activation and diminishes the immune response.

Clone

mB7-2.

Immunogen

Purified mouse LPS-activated B cells.

Ig Subclass

Rat IgG2a.

Purification Method

Protein-A.

Protein concentration

1mg/ml in PBS (after reconstitution).

Storage Procedures

Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.

Reconstitution

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C.

Antibody Properties

Blocking and staining antibody. For staining, use 10µl/106 cells. Titer for blocking T cell activation should be determined by the investigator.

Available Conjugates

This antibody is only available conjugated to Biotin and FITC.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.