CD28 co-stimulation is necessary for CD4 positive T-cell proliferation & survival, interleukin-2 production, and T-helper type-2 development. Human post-thymic regulatory T cells require CD28 co-stimulation to expand and maintain potent suppressive function in vivo. Apoptosis plays a key role in the age-related decline of CD28 expression and in immunosenescence.

Clone

NYRmCD28.

Immunogen

Purified mouse purified mouse LN T cells.

Ig Subclass

Hamster IgG.

Purification Method

Protein-A.

Protein concentration

1mg/ml in PBS (after reconstitution).

Storage Procedures

Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.

Reconstitution

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C.

Antibody Properties

Blocking and staining antibody. For staining, use 10µl/106 cells. Titer for blocking T cell activation should be determined by the investigator.

Available Conjugates

This antibody is only available non-conjugated to Biotin.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.