Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.
i Cells successfully cultured in Advanced DMEM ⁄ F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2.
This Advanced MEM is manufactured as follows:
With | Without |
• Glucose | • L-glutamine |
• Non Essential Amino Acids | • HEPES |
• Sodium pyruvate | |