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订购正品MBIER1581;AarI现货

  • 进口
    产地:MBI 
    货号:ER1581

    Conditions for 100% Activity
    [1X Buffer AarI] + oligonucleotide:
    [10 mM Bis-Tris Propane-HCl (pH 6.5 at 37°C), 10 mM MgCl2, 100 mM KCl, 0.1 mg/ml BSA] + 0.5 µM of oligonucleotide (see Note).
    Incubate at 37°C.
    Digestion of Agarose-embedded DNA
    Minimum 5 units of the enzyme are required for digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.
    Storage Buffer
    AarI is supplied in:
    10 mM potassium phosphate (pH 7.4 at 25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA and 50% (v/v) glycerol.
    Ligation and Recleavage
    After 10-fold overdigestion with AarI, more than 95% of the DNA fragments can be ligated and recut.
    ER1581 25 u (2 u/µl) 1.00 ml 1.00 ml 25.00 µl ER1581  
    ER1582 125 u (2 u/µl) 1.00 ml 1.00 ml 2 x 25.00 µl ER1582