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订购MBIER0991;AatII现货原装

  • 进口
    产地:MBI 

    货号:ER0991


    Conditions for 100% Activity

    1X Buffer Tango™:

    33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1mg/ ml BSA.

    Incubate at 37°C.

    Storage Buffer

    AatII is supplied in:

    10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM DTT, 1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.

    Digestion of Agarose-embedded DNA

    Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours.

    Ligation and Recleavage

    After 50-fold overdigestion with AatII, more than 95% of the DNA fragments can be ligated and recut.