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大量现货|LysingEnzymes|溶壁酶|Lyticase|CAS37340-57-1

  • 进口
  • Sigma
    产地:usa
    货号:L2524
    Lyticase from Arthrobacter luteus
    CAS号: 37340-57-1

    Application
    Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
    Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
    相关类型 Application Index, Carbohydrate Hydrolysis, Carbohydrate hydrolysis & PTM analysis, Cell Dissociation and Cell Lysis, Cell Lysis Reagents,
    Cell Lysis and Protein Extraction Reagents, Enzymatic Cell Lysis and Protoplast Preparation, Enzymes, Enzymes for Cell Lysis, Enzymes, Inhibitors, and Substrates, Lysing Enzymes, Lysing Enzymes and Protoplast Preparation, Molecular Biology, Molecular Biology Enzymes, Native Protein Sample Preparation, Signal Transduction Research Tools, Yeast
    more...
    Quality Level PREMIUM
    form lyophilized powder
    composition Protein, ≥20% biuret 
    storage temp. −20°C
    Unit Definition
    One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
    Physical form
    Partially purified, lyophilized powder containing potassium phosphate buffer salts and stabilizers
    本产品仅用于生命科学研究实验。