ACATAntibody

ACAT Antibody
Cat.#: 1864-1
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P23141
Mol Weight: 55kDa
Size: 100ul

Description
Acyl-CoA:cholesterol acyltransferase (ACAT) is an integral membrane enzyme that belongs to the type-B carboxylesterase/lipase family. ACAT catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty-acyl-coenzyme A (1). ACAT also function as a regulator of intracellular cholesterol homeostasis at a single cell level. Two isoforms of ACAT, ACAT1 and ACAT2, are expressed in the liver and intestine. However, ACAT1 is only found in macrophages and SMCs (2). The accumulation of cholesteryl esters produced by ACAT in macrophages can lead to foam cell formation, an indication of early stage atherosclerosis. ACAT activity has been linked to Alzheimer's Disease, a condition caused by excess amount of amyloid beta-peptide (Abeta). Atherogenic lipoproteins activate ACAT activity, where active ACAT can modulate the level of Abeta (3).

Recommended Applications
WB, IHC, ICC

Applications and Recommended Dilution Factors
WB: 1:500
IHC: 1:100-1:250
ICC: 1:100-1:250

Species Reactivity
Human, Mouse, Rat

Cross reactivity determined by western blot only.

Products Data 

 

A. Western blot analysis on fetal liver using anti-ACAT RabPAb (Cat # 1864-1), dilution 1:500.
B. Immunohistochemical analysis of paraffin-embedded human liver using anti-ACAT RabPAb (Cat# 1864-1).

Specificity
A synthetic peptide corresponding to residues in human ACAT was used as immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
CES1 antibody, ACAT antibody, CEH antibody, CES2 antibody, HMSE antibody, HMSE1 antibody, MGC117365 antibody, PCE-1 antibody, SES1 antibody, TGH antibody, Liver carboxylesterase 1 antibody, Serine esterase 1 antibody, Triacylglycerol hydrolase antibody

Description References
1. Buhman KF et al. Biochim Biophys Acta 1529(1-3):142-54, 2000