Alpha-Actinin1(ACTN1)antibody

Alpha-Actinin 1(ACTN1)antibody
Cat.#: 2358-1
Rabbit Monoclonal Antibody
Clone ID: EP2528Y
Swiss Prot: P12814
Mol Weight: 100kDa
Size: 100ul

Description
Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. Alpha-actinin-1 and -4 (ACTN1, ACTN4) are non-muscle isoforms version of ACTN. These isoforms are commonly expressed in filopodia, lamellipodia, stress fibers, sites of adhesion and intercellular contacts (2).

Recommended Applications
WB, ICC, IP, FC

Applications and Recommended Dilution Factors
WB: 1:500 - 1000
ICC: 1:100-250
IP: 1:40
FC: 1:40

Species Reactivity
Human

Cross reactivity for this product was determined by western blot.

Products Data

A. Western blot analysis on (A) NIH 3T3 (B) human skeletal muscle tissue (C) human heart tissue lysates using anti-alpha-actinin 1 RabMAb (cat.# 2358-1), dilution 1:1000.

Specificity
A synthetic peptide corresponding to residues on the C-terminus of human Alpha-Actinin 1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ACTN1 antibody, FLJ40884 antibody, FLJ54432 antibody, Alpha-actinin-1 antibody, Alpha-actinin cytoskeletal isoform antibody, F-actin cross-linking protein antibody, Non-muscle alpha-actinin-1 antibody

Description References
1. Ylanne J, et al. Structure 9(7):597-604, 2001
2. Otey, CA and Carpen O. Cell Motil. Cytoskeleton 58:104-111, (2004).