AE1Phospho(pY21)(SLC4A1)antibody

AE1 Phospho(pY21)(SLC4A1)antibody
Cat.#: 3557-1
Rabbit Monoclonal Antibody
Clone ID: EPR1424
Swiss Prot: P02730
Mol Weight: 100kDa
Size: 100ul

Description
The AE1 (anion exchanger, band 3) protein is expressed in erythrocytes and in the A-type intercalated cells of the kidney distal collecting tubule. In both cell types it mediates the electroneutral transport of chloride and bicarbonate ions across the lipid bilayer, and, in erythrocytes (1). AE1 is phosphorylated on Tyr8and Tyr21 by SYK. It has been suggested that, upon phosphorylation by p72syk, Tyr8, and Tyr21 act as docking sites for the SH2 domain of Lyn, which subsequently phosphorylates AE1 at additional secondary sites (2).

Recommended Applications
WB

Applications and Recommended Dilution Factors
WB: 1:50,000 - 200,000

Species Reactivity
Human

Species cross-reactivity determined by WB analysis only

Products Data

Western blot analysis on red blood cell lysates using anti-Phospho-AE1 (pY21) RabMAb (cat. #3557-1). Cells were either (A) untreated (B) treated with pervanadate.

Specificity
A phospho specific peptide corresponding to residues surrounding tyrosine 21 of human AE1 was used as an immunogen. This antibody only detects AE1 phosphorylated at tyrosine 21.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
SLC4A1 antibody, AE1 antibody, BND3 antibody, CD233 antibody, DI antibody, EMPB3 antibody, EPB3 antibody, FR antibody, MGC116750 antibody, MGC116753 antibody, MGC126619 antibody, MGC126623 antibody, RTA1A antibody, SW antibody, WD antibody, WD1 antibody, WR antibody, Band 3 anion transport protein antibody, Solute carrier family 4 member 1 antibody

Description References
1. Sahr KE, et al. Genomics 24(3):491-501, 1994
2. Brunati AM, et al. Blood 96(4):1550-7, 2000