AMPKalpha1Phospho(pT183)/AMPKalpha2Phospho(pT172)(PRKAA1)antibody

AMPK alpha 1 Phospho(pT183)/AMPK alpha 2 Phospho(pT172)(PRKAA1)antibody
Cat.#: 3930-1
Rabbit Monoclonal Antibody
Clone ID: EPR5683
Swiss Prot: Q13131
Mol Weight: 62/63kDa
Size: 100ul

Description
The 5-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose nonfermentor) kinase family (1) AMPK is a heterotrimeric protein comprising α (63 kDa), β (38 kDa) and γ (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are noncatalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.

Recommended Applications
WB, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IP: 1:10 - 100

Species Reactivity
Human, Mouse, Rat

Cross reactivity determined by western blot only.

Products Data

Western blot analysis on 293 cell lysates using anti-Phospho-AMPK alpha 1/AMPK alpha 2 (pT183/pT172) RabMAb (cat. #3930-1). Cells were either (A) untreated or (B) treated with Lambda phosphatase.

Specificity
A phospho specific peptide corresponding to residues surrounding Threonine 183 of human AMPK alpha 1 and Threonine 172 of human AMPK alpha 2 was used as an immunogen. This antibody only detects AMPK alpha 1 phosphorylated at Threonine 183 and AMPK alpha 2 phosphorylated Threonine 172.

Storage Condition and Buffer
Store at -20° C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
PRKAA1 antibody, AMPK antibody, AMPKa1 antibody, MGC33776 antibody, MGC57364 antibody, 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody, antibody

Description References
1. Mitchelhill, K. I., et al. J. Biol. Chem. 269, 2361-2364, 1994
2. Stapleton et al. J. Biol. Chem. 271:611-614
3. Hamilton et al. FEBES Lett. 500:163-168, 2001
4. Carling, D., et al. Eur. J. Biochem. 186, 129-136, 1989