HC Class II is consists of 2 membrane spanning proteins. Each of these proteins is roughly 30 kDa, and has of two Globular domains, Alpha-1, Alpha-2, Beta-1 and Beta-2. The two regions farthest away from the membrane are alpha-1 and beta-1. The two proteins link without Covalent Bonds. The MHC Class II protein mainly presents peptides, which have been digested from external sources. MHC Class II is normally only expressed by Antigen Presenting Cells (APC) which digest foreign protein. Within the RER the alpha and beta proteins of the molecule, associate with each other, while a third protein called the "invariant chain," is necessary for stabilizing the complex, without it, they will not associate. The MHC-invariant complex is then passed from the RER, into and out of, the Golgi body. It fuses with an Endocytic compartment, where an external protein has been sampled and degraded.

Clone

NYRmI-E.

Immunogen

Purified mouse LN B cells (C3H anti-C57Bl6).

Ig Subclass

Mouse IgG2a.

Specificity

Recognizes most MHC I-E class II haplotypes Reacts with H-2k, H-2d, H-2p and H-2r.

Purification Method

Ion exchange column.

Protein concentration

1mg/ml in PBS (after reconstitution).

Storage Procedures

Lyophilized: store at 4C. After reconstitution, if not intended for use within a month, aliquot and store at -20C.

Reconstitution

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Stability / Shelf Life

Two years lyophilized, one month in solution at 4C.

Antibody Properties

Cytotoxic and staining antibody. For staining, use 10 ml/106 cells. Titer for cytotoxicity should be determined by the investigator.

Available Conjugates

This antibody is also available as unconjugated antibody and conjugated to FITC.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.