Acylproteinthioesterase1(LYPLA1)antibody

Acyl protein thioesterase 1(LYPLA1)antibody
Cat.#: 2650-1
Rabbit Monoclonal Antibody
Clone ID: EPR3667
Swiss Prot: O75608
Mol Weight: 24/21kDa
Size: 100ul

Description
Acyl-protein thioesterase 1 (APT1, Lysophospholipase 1) is a member of a family of critical enzymes, lysophospholipases, that act on biological membranes to regulate the multifunctional lysophospholipids (1). APT1 hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS (2). The role of APT1 in regulating lysophospholipids is important because increased levels of lysophospholipids are detected in a variety of diseases, including atherosclerosis and hyperlipidemia (1). Regulated expression of acyl-protein thioesterase by PKC has been suggested to have important implications for signal transduction processes (3).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 - 250
ICC: 1:500 - 1000
IP: 1:10

Species Reactivity
Human, Mouse, Rat

Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data 

 

A. Western blot analysis on (A) fetal liver, (B) fetal heart, (C) human spleen, (D) mouse heart, (E) mouse kidney, (F) mouse spleen and (G) rat kidney lysates using anti-Acyl protein thioesterase 1 RabMAb (cat. #2650-1).
B. Immunohistochemical analysis of paraffin-embedded human brain using anti-Acyl protein thioesterase 1 RabMAb (cat. #2650-1).

Specificity
A synthetic peptide corresponding to residues in human Acyl protein thioesterase 1 was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
LYPLA1 antibody, APT-1 antibody, LPL1 antibody, LYSOPLA antibody, hAPT1 antibody, Acyl-protein thioesterase 1 antibody, Lysophospholipase 1 antibody, Lysophospholipase I antibody

Description References
1. Wang A, et al. Biochim Biophys Acta. 1437(2):157-69, 1999 2. The UniProt Consortium, The Universal Protein Resource (UniProt). Nucleic Acids Res., 36:D190-D195 (2008) 3. Wang A, et al. Biochim Biophys Acta. 1484(2-3):207-14, 2000