ApolipoproteinE(apoE)(APOE)antibody

Apolipoprotein E(apo E)(APOE)antibody
Cat.#: 1930-1
Rabbit Monoclonal Antibody
Clone ID: EP1374Y
Swiss Prot: P02649
Mol Weight: 36kDa
Size: 100ul

Description
The apolipoproteins are family of amphipathic lipoproteins that plays a crucial role in lipid metabolism. In particular, apolipoprotein E (apo E) is a major component of high density lipoproteins (HDL) chylomicrons and very low-density lipoprotein (VLDL), which functions to remove excess cholesterol. Apo E mediates lipid metabolism and cholesterol homeostasis by binding to several hepatic lipoprotein receptors, such as low density lipoprotein receptor (LDLR) and the LDLR-related protein (1). Apo E deficiency is linked to hyperlipoproteinemia type III; a genetic disorder characterized by elevated plasma cholesterol and triglyceride levels and accelerated coronary artery disease (2). Three major isoforms of Apo E (E2, E3, E4) has been identified, with E3 being the most common in human. E4 presence has been associated to increase probability of developing late-onset form of Alzheimer disease (3).

Recommended Applications
WB, IHC, ICC, IP

Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:100 250
ICC: 1:250 500
IP: 1:100

Species Reactivity
Human, Rat

For Mouse, this antibody is recommended for WB only
Rat cross reactivity tested by western blot only

Products Data  

 

A. Western blot analysis on human serum using anti-Apo E Rabbit Monoclonal. (Cat. # 1930-1).
B. Immunohistochemical analysis of paraffin-embedded human normal liver using anti-Apo E RabMAb (1930-1).
C. Immunofluorescent staining of HEP G2 cells using anti- Apo E RabMAb (1930-1).

Specificity
A synthetic peptide corresponding to residues near the C-term of human apolipoprotein E was used as an immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
APOE antibody, AD2 antibody, LDLCQ5 antibody, LPG antibody, MGC1571 antibody, Apolipoprotein E antibody, antibody

Description References
1. Zaioua M et al, Journal of Lipid Research, 41:1087-1095, 2000
2. Mahley RW and SC Rall, Jr. 7th ed. McGraw-Hill, New York. 1995.
3. Olarte L et al, Arch Neurol. 63(11):1586-90, 2006

Product References
1. Jesse S, Steinacker P, Lehnert S, et al, A Proteomic Approach for the Diagnosis of Bacterial Meningitis ,PLoS One 5 (2010) e10079
[Application: WB]