ATP-citratelyase(C-term)protein(ACLY)antibody

ATP-citrate lyase(C-term)protein(ACLY)antibody
Cat.#: 1699-1
Rabbit Monoclonal Antibody
Clone ID: EP704Y
Swiss Prot: P53396
Mol Weight: 125kDa
Size: 100ul

Description
ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL (2).

Recommended Applications
WB, IP, FC

Applications and Recommended Dilution Factors
WB: 1:1,000-10,000
IP: 1:50
FC: 1: 30

Species Reactivity
Human, Mouse, Rat

Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on Hela cell lysate using anti-ATP citrate lyase RabMAb (cat. # 1699-1).

Specificity
A synthetic peptide corresponding to residues in the C-term of human ATP citrate lyase was used as immunogen.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ACLY antibody, ACL antibody, ATPCL antibody, CLATP antibody, ATP-citrate synthase antibody, Citrate cleavage enzyme antibody

Description References
1. Elshourbagy et al. Europ. J. Biochem. 204: 491-499, 1992.
2. Benjamin, W. B., and Singer, I. (1974) Biochim. Biophys. Acta 251, 28-42

Product References
1. King FW, Fong S, Griffin C, et al, Timosaponin AIII Is Preferentially Cytotoxic to Tumor Cells through Inhibition of mTOR and Induction of ER Stress ,PLoS One 4 (2009) e7283
[Application: WB]
2. Hasan NM, Longacre MJ, Seed Ahmed M, et al, Lower succinyl-CoA:3-ketoacid-CoA transferase (SCOT) and ATP citrate lyase in pancreatic islets of a rat model of type 2 diabetes: Knockdown of SCOT inhibits insulin release in rat insulinoma cells ,Arch Biochem Biophys 499 (2010) 62-68
[Application: WB]