ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling.

Clone

mCD11a.

Immunogen

Enriched mouse peritoneal macrophages.

Ig Subclass

Rat IgG2b.

Purification Method

Ion exchange column.

Protein concentration

1mg/ml in PBS (after reconstitution).

Storage Procedures

Lyophilized: store at 4oC. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.

Reconstitution

Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC.

Antibody Properties

Staining and blocking antibody. For staining, use 10 µl/106 cells. This antibody will block signaling through the CD11a molecule. Titer for blocking should be determined by the investigator.

Usage

Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.