Tumor necrosis factor is a cytokineinvolved in systemic inflammationand is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptoticcell death, cellular proliferation, differentiation, inflammation, tumorigenesisand viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.

Clone

NYRhTNFa-E2.

Immunogen

r.Human TNF-a.

Ig Subclass

Mouse IgG1.

Purification Method

ion exchange.

Protein concentration

1mg/ml in PBS (after reconstitution).

Shipping Conditions

Antibody is shipped lyophilized at ambient temperature.

Storage Procedures

In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.

Reconstitution

Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC.

Titer

In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories) 1:10,000 dilution will yield 0.7 O.D within 10 minutes.

Applications

Direct ELISA, Western Blot, Immuneprecipitation, Intracellular staining.

Note

This antibody will bind very well to protein A in a buffer (PBS) containing high salt concentration (3M Nacl).

Usage

Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.