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MouseAnti-HumanMacrophageInflammatoryProtein-1a(CCL3/MIP-1a)

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  • Prospec
    Catalogue Number ANT-118
    Synonyms Small inducible cytokine A3, CCL3, Macrophage inflammatory protein 1-alpha, MIP-1-alpha, Tonsillar lymphocyte LD78 alpha protein, G0/G1 switch regulatory protein 19-1, G0S19-1 protein, SIS-beta, PAT 464.1, chemokine (C-C motif) ligand 3, MIP1A, SCYA3, G0S19-1, LD78ALPHA.
    Type Mouse Anti Human Monoclonal.
    Introduction Macrophage Inflammatory Proteins (MIP) belong to the family of chemotactic cytokines known as chemokines. In humans, there are two major forms, MIP-1? and MIP-1? that are now officially named CCL3 and CCL4 respectively. Both are major factors produced by macrophages after they are stimulated with bacterial endotoxins. They activate human granulocytes (neutrophils, eosinophilsand basophils) which can lead to acute neutrophilic inflammation. They also induce the synthesis and release of other pro-inflammatory cytokines such as interleukin 1(IL-1), IL-6 and TNF-?from fibroblasts and macrophages. The genes for CCL3 and CCL4 are both located on human chromosome 17.
    Clone YNR-HMIP1a.
    Immunogen r.Human MIP1-a.
    Ig Subclass Mouse IgG1.
    Purification Method Ion exchange.
    Protein concentration 1mg/ml in PBS (after reconstitution).
    Shipping Conditions Antibody is shipped lyophilized at ambient temperature.
    Storage Procedures In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.
    Reconstitution Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC.
    Applications Direct ELISA, Western Blot, Immuneprecipitation.
    Titer By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories) UsageProspec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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