ADH5Antibody(Center)

ADH5 Antibody(Center)
Cat.#: T3254
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: P11766
Mol Weight: 40kDa
Size: 100ug

Description
ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis.

Recommended Applications
WB, IHC, FC, ELISA

Applications and Recommended Dilution Factors
WB: 1:100~500
IHC: 1:50~100
FC: 1:10~50
ELISA: 1:1,000

Species Reactivity
Human

Products Data

 

Western blot analysis of ADH5 Antibody (Center) in K562 cell line lysates (35ug/lane). ADH5 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADH5 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specificity
This ADH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 219~248 amino acids from the Center region of human ADH5.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Alternative Names
ADH5 antibody, ADH-3 antibody, ADHX antibody, FDH antibody, GSNOR antibody, Alcohol dehydrogenase class-3 antibody, S-(hydroxymethyl)glutathione dehydrogenase antibody

Description References
Martins-de-Souza,D., et.al., Eur Arch Psychiatry Clin Neurosci 259 (3), 151-163 (2009)
Iborra,F.J., et.al., J. Histochem. Cytochem. 40 (12), 1865-1878 (1992)