AlkPhospho(pY1604)(ALK)antibody

Alk Phospho(pY1604)(ALK)antibody
Cat.#: 1891-1
Rabbit Monoclonal Antibody
Clone ID: EP661Y
Swiss Prot: Q9UM73
Mol Weight: 220kDa
Size: 100ul

Description
ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. The 2;5 chromosomal translocation occurs in most anaplastic large-cell non-Hodgkin's lymphomas arising from activated T lymphocytes. This rearrangement was shown to fuse the NPM nucleolar phosphoprotein gene on chromosome 5q35 to a previously unidentified protein tyrosine kinase gene, ALK, on chromosome 2p23 (1). In the predicted hybrid protein, the amino terminus of NPM is linked to the catalytic domain of ALK. Immunoblotting with anti-ALK antibody shows that ALK is highly expressed in the neonatal brain (2). Also expressed in the small intestine, testis, and brain but not in normal lymphoid cells, ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases. Unscheduled expression of the truncated ALK may contribute to malignant transformation in these lymphomas (3).

Recommended Applications
WB, IHC, IP

Applications and Recommended Dilution Factors
WB: 1:5000
IHC: 1:100 - 250
IP: 1:20

Species Reactivity
Human

Cross reactivity determined by western blot only.

Products Data 

 

A. Western blot analysis on SH-SY-5Y cell lysates using anti-Phospho-ALK (pY1604) RabMAb (cat. #1891-1), 1:5000 dilution. Cells were either (A) untreated (B) treated with PTN
B. Immunohistochemical analysis of human Non-Hodgkins lymphoma using anti-Phospho-ALK (pY1604) RabMAb (cat. # 1891-1).

Specificity
A synthetic phosphor-peptide corresponding to residues surrounding Tyr1604 of human ALK was used as an immunogen. The antibody only detects ALK phosphorylated on Tyrosine 1604.

Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Alternative Names
ALK antibody, CD246 antibody, NBLST3 antibody, ALK tyrosine kinase receptor antibody, Anaplastic lymphoma kinase antibody

Description References
1. Morris SW, et al. Science 263(5151):1281-4, 1994.
2. Iwahara T, et al. Oncogene 14(4):439-49, 1997.
3. Benharroch D, et al. Blood 91(6): 2076-84, 1998.