网站首页 资讯 仪器谱 耗材谱 试剂谱 人才 论坛 搜索 移动检测 博客登录

DNeasyPlantMiniKit现货供应

  • 进口
  • qiagen
    货号 产品名称 厂家 规格 单价 详细 订购
    69104 DNeasy Plant Mini Kit (50) Qiagen 50T ¥2,310.00 详细查看 订购
    货号:69104
    产地:qiagen
    DNeasy Plant Mini Kit
    植物细胞,组织或真菌细胞样品分离总DNA,最高产量可达 30 µg
    纯DNA,无污染物和酶抑制剂
    快速纯化得到即用型DNA
    无需有机抽提,无需乙醇沉淀
    Format: Mini spin columns
    with 2 ml collection tubes
    Sample source: Plant cells and tissues, fungi
    Sample size: Up to 100 mg wet weight
    Preparation time: <1 hour
    Typical yield:* 3–30 µg
    Elution volume: 50–400 µl
    Procedure
    Samples are first mechanically disrupted and then chemically lysed. RNA is removed by RNase digestion during lysis. Cell debris is removed and samples are filtered and homogenized by centrifugation through a QIAshredder spin column. Buffering conditions are adjusted, precipitating proteins and polysaccharides, and the lysate is loaded onto the DNeasy Plant spin column. During a brief spin, DNA selectively binds to the silica-gel membrane while contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use (see flowchart "DNeasy Plant and DNeasy 96 Plant Procedures").
    Downstream applications
    The DNeasy Plant procedure yields pure nucleic acid, free of polysaccharides and other secondary metabolites often copurified using conventional methods. Such impurities can interfere with spectrophotometric readings and inhibit enzymatic reactions. DNeasy purified DNA is sized up to 40 kb (see figure "Pure DNA (20–25 kb) for Restriction Analysis"), and is suitable for downstream applications such as:
    PCR (see figure "PCR Analysis of DNA from Different Plant Species")
    AFLP
    RFLP
    RAPD (see figure "RAPD Analysis of Sunflower Species")
    Southern blotting
    Microsatellite analysis
    Real-time PCR
    Cited References
    Dellaporta, S.L. (1983) A plant DNA minipreparation: version II. Plant Mol. Biol. Rep. 1, 19.
    Doyle, J.J. and Doyle J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19, 11.
    订购电话:021-51870956
    在线QQ:470003480
    快来
    www.haoranbio.com体验在线订购给您带来的快捷和方便吧!