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MBIEP0161;TerminalDeoxynucleotidylTransferase现货热销

  • 进口
    产地:MBI 

    货号:EP0161


    Applications

    Production of synthetic homo- and heteropolymers (1).

    Homopolymeric tailing of linear duplex DNA with any type of 3'-OH terminus (2, 3).

    Oligodeoxyribonucleotide and DNA labeling (2, 4-8).

    5'-RACE (Rapid Amplification of cDNA Ends) (9).

    In situ localization of apoptosis (10).

    Description

    Terminal Deoxynucleotidyl Transferase (TdT), a template-independent DNA polymerase, catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded DNA (1). TdT requires an oligonucleotide of at least three nucleotides to serve as a primer. With RNA as template TdT shows variable performance which strongly depends upon the tertiary structure of acceptor RNA 3'-end and the nature of nucleotide. Generally, it is lower than using DNA as a template.



















    EP0161

    500 u (20 u/µl)

    0.40 ml

    EP0161

     

    EP0162

    2500 u (20 u/µl)

    2 x 1.00 ml

    EP0162