Akt1 Phospho(pS473)(AKT1)antibody
Cat.#: 2118-1
Rabbit Monoclonal Antibody
Clone ID: EP2109Y
Swiss Prot: P31749
Mol Weight: 60kDa
Size: 100ul
Description
Akt1/PKBa is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBa is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBa inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBa regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBa isoforms have been identified.
Recommended Applications
WB, IHC, ICC
Applications and Recommended Dilution Factors
WB: 1:1000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
Species Reactivity
Human, Mouse
Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only
Products Data
Specificity
A phospho specific peptide corresponding to residues surrounding Ser473 of human Akt1 was used as immunogen. The antibody detects Akt1 phosphorylated at Serine 473.
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Alternative Names
AKT1 antibody, AKT antibody, MGC99656 antibody, PKB antibody, PKB-ALPHA antibody, PRKBA antibody, RAC antibody, RAC-ALPHA antibody, RAC-alpha serine/threonine-protein kinase antibody, Proto-oncogene c-Akt antibody, RAC-PK-alpha antibody
Description References
1. Burgering, B.M. and P.J. Coffer.Nature 376: 599
2. Cardone, M.H., et al. Science 282: 1318
3. Hajduch, E., et al. FEBS Lett. 492: 199
4. Cross, D.A., et al. Nature 378: 785-789 (1995).
Product References
1. Renner O, Blanco-Aparicio C, Grassow M, et al, Activation of Phosphatidylinositol 3-Kinase by Membrane Localization of p110α Predisposes Mammary Glands to Neoplastic Transformation ,Cancer Res 68 (2008) 9643-9653
[Application: IHC]
2. Blanco-Aparicio C, Cañamero M, Cecilia Y, et al, Exploring the Gain of Function Contribution of AKT to Mammary Tumorigenesis in Mouse Models ,PLoS One 5 (2010) e9305
[Application: IHC]
3. Pommier AJ, Alves G, Viennois E, et al, Liver X Receptor activation downregulates AKT survival signaling in lipid rafts and induces apoptosis of prostate cancer cells ,Oncogene 29 (2010) 2712-2723
[Application: ICC]
4. Kunoh T, Noda T, Koseki K, et al, A Novel Human Dynactin-Associated Protein, dynAP, Promotes Activation of Akt, and Ergosterol-Related Compounds Induce dynAP-Dependent Apoptosis of Human Cancer Cells ,Mol Cancer Ther 9 (2010) 2934-2942