ACO2Antibody(Center)

ACO2 Antibody(Center)
Cat.#: T0502
Rabbit Polyclonal Antibody
Clone ID: polyclonal
Swiss Prot: Q99798
Mol Weight: 85kDa
Size: 100ug

Description
ACO2 belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification.

Recommended Applications
WB, IHC, ELISA

Applications and Recommended Dilution Factors
WB: 1:100~500
IHC: 1:10~50
ELISA: 1:1,000

Species Reactivity
Human, Mouse, Rat

Products Data  

 

Western blot analysis of anti-ACO2 Pab in mouse heart(left) and 293 (right) tissue lysates (35ug/lane). ACO2(arrow) was detected using the purified Pab (1:60 dilution).
Perfused isolated rat heart whole tissue lysate was lysed with either A) 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% NP-40, 0.1% SDS, 0.5% Na-deoxycholate, 1 mM Na3VO4, 20 mM NaF, 1 mM PMSF, 5 v/v % protease inhibitor cocktail or B) T-PER Tissue Protein Extraction Reagent [# 785101; Pierce], containing 1mM Na3VO4, 20 mM NaF, 5 v/v % protease inhibitor cocktail (Sigma); PVDF membrane was incubated in primary Ab [rabbit polyclonal antibody against ACO2 (Center) . Solution: 1:1000 diluted in 5% NFM TBS-T 0,05 for overnight (15 hrs) at 4 ?? Data courtesy of Boglarka Laczy M.D., Division of Cardiovascular Disease, Dept. of Medicine, University of Alabama at Birmingham.
ACO2 western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Aconitase antibody detected the Aconitase protein (arrow).

Specificity
This ACO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445~475 amino acids from the central region of human Aconitase.

Storage Condition and Buffer
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Alternative Names
ACO2 antibody, ACONM antibody, MGC20605 antibody, MGC33908 antibody, Aconitate hydratase, mitochondrial antibody, Citrate hydro-lyase antibody

Description References
Juang, H.H., Mol. Genet. Metab. 81(3):244-252 (2004).
Bota, D.A., et al., Nat. Cell Biol. 4(9):674-680 (2002).
Gruer, M.J., et al., Trends Biochem. Sci. 22(1):3-6 (1997).
Klausner, R.D., et al., Mol. Biol. Cell 4(1):1-5 (1993).
Geurts van Kessel, A.H., et al., Cytogenet. Cell Genet. 28(3):169-172 (1980).