| Catalogue Number | ANT-209 |
| Type | Rat Anti Mouse Monoclonal. |
| Introduction | IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity. |
| Clone | NYRmIL-12p75. |
| Immunogen | r.Murine IL-12 p75. |
| Ig Subclass | Rat IgG2b. |
| Purification Method | Ion exchange. |
| Protein concentration | 1mg/ml in PBS (after reconstitution). |
| Shipping Conditions | Antibody is shipped lyophilized at ambient temperature. |
| Storage Procedures | In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC. |
| Reconstitution | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC. |
| Applications | Direct ELISA, Western Blot, Immuneprecipitation. |
| Titer | By direct ELISA, 1:5,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
| Note | This antibody recognizes the entire IL-12 molecule and has no reactivity to denatured p40 or p35. |
| Usage | Prospec's products are furnished for LABORATORY ESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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