网站首页 资讯 仪器谱 耗材谱 试剂谱 人才 论坛 搜索 移动检测 博客登录

AMVReverseTranscriptase,10u/ul

  • 进口



















    Avian Myeloblastosis Virus Reverse Transcriptase (AMV RT) catalyzes the polymerization of DNA using template DNA, RNA or RNA:DNA hybrids (1). It requires a primer (DNA primers are more efficient than RNA primers) as well as Mg2+ or Mn2+.






































    Cat No

    Size

    Price($)


    A1201-200

    200 units

    75


    A1201-500

    500 units

    155















     

     




















    The enzyme possesses an intrinsic RNase H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.


     


    Applications

    - First- and second-strand synthesis of cDNA.

    - Primer extensions and RNA sequencing

    - RT-PCR(a). Up to 10μl of an RT reaction containing AMV RT and the supplied AMV RT Reaction Buffer can be added to a 50μl PCR amplification reaction that uses Taq DNA polymerase.